N Cadherin Recombinant Rabbit Monoclonal Antibody [SY02-46] (ET1607-37)
Catalog# ET1607-37
Rabbit Monoclonal to N Cadherin
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Lane 1: 293T cell lysate
Lane 2: A549 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A-172 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 140-150 kDa
Exposure time: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-37) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Safety datasheet
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- No MSDS Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
N Cadherin Recombinant Rabbit Monoclonal Antibody [SY02-46] (ET1607-37)
Immunogen
Synthetic peptide within human n cadherin aa 161-210 / 906.
Host
Rabbit
Positive Control
293T cell lysate, A549 cell lysate, HeLa cell lysate, A-172 cell lysate, MCF7 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse liver tissue, rat liver tissue, human liver carcinoma tissue, human liver tissue, mouse heart tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY02-46
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IHC-P
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1:10,000-1:40,000
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IHC-Fr
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1:500
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IF-Tissue
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 100 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
N Cadherin
SYNONYMS
CADH2_HUMAN antibody;Cadherin 2 antibody;Cadherin 2 N cadherin neuronal antibody;Cadherin 2 type 1 antibody;Cadherin 2 type 1 N cadherin neuronal antibody;Cadherin 2, type 1, N-cadherin (neuronal) antibody;Cadherin-2 antibody;Cadherin2 antibody;Calcium dependent adhesion protein neuronal antibody;CD325 antibody;CD325 antigen antibody;CDH2 antibody;CDHN antibody;CDw325 antibody;CDw325 antigen antibody;N cadherin 1 antibody;N-cadherin antibody;NCAD antibody;Neural cadherin antibody;OTTHUMP00000066304 antibody;OTTHUMP00000067378 antibody
POST-TRANSLATIONAL MODIFICATION
Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).; May be phosphorylated by OBSCN.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.
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