MYH7B Recombinant Rabbit Monoclonal Antibody [JE54-44] (ET7110-69)
Catalog# ET7110-69
Rabbit Monoclonal to MYH7B
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WB
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Rat
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Mouse
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Human
Lane 1: Rat heart tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 226 kDa
Observed band size: 226 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MYH7B Recombinant Rabbit Monoclonal Antibody [JE54-44] (ET7110-69)
Immunogen
Recombinant protein within human myh7b aa 1,710-1,983 / 1,983.
Host
Rabbit
Positive Control
Rat heart tissue lysate, Rat skeletal muscle tissue lysate, Mouse skeletal muscle tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE54-44
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 226 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MYH7B
SYNONYMS
Antigen MLAA-21 antibody;cardiac muscle beta isoform antibody;Myh7b antibody;MYH7B_HUMAN antibody;Myosin cardiac muscle beta chain antibody;Myosin heavy chain 7B antibody;Myosin-7B antibody;Slow A MYH14 antibody
SEQUENCE SIMILARITIES
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
TISSUE SPECIFICITY
Expressed in heart, skeletal muscle, testis, and all specific brain regions examined. Slightly lower expression was detected in ovary and kidney, and intermediate expression was detected in lung, pancreas, spleen and liver.
DEVELOPMENTAL STAGE
Found in fetal liver and brain.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Myosin-7B also known as myosin, heavy chain 7B is a protein that in humans is encoded by the MYH7B gene.MYH7B is a slow-twitch myosin.The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined.
CITATIONS
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Punicalagin protects against impaired skeletal muscle function in high-fat-diet-induced obese mice by regulating TET2
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WB
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Mouse
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Citation
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