PRODUCT CODE: ET7110-69

MYH7B Recombinant Rabbit Monoclonal Antibody [JE54-44] (ET7110-69)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Rat

  • Human

  • Mouse

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+
Western blot analysis of MYH7B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: rat skeletal muscle tissue lysate<br />
Lane 2: rat heart tissue lysate
  • Western blot analysis of MYH7B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: rat skeletal muscle tissue lysate<br />
Lane 2: rat heart tissue lysate
Western blot analysis of MYH7B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: rat skeletal muscle tissue lysate
Lane 2: rat heart tissue lysate

Applications

  • WB

REACTIVITY

  • Rat

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MYH7B Recombinant Rabbit Monoclonal Antibody [JE54-44] (ET7110-69)

Immunogen

Recombinant protein within human myh7b.

Host

Rabbit

Positive Control

Rat skeletal muscle tissue lysate, rat heart tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE54-44

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

226 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

MYH7B

SYNONYMS

Antigen MLAA-21 antibody; cardiac muscle beta isoform antibody; Myh7b antibody; MYH7B_HUMAN antibody; Myosin cardiac muscle beta chain antibody; Myosin heavy chain 7B antibody; Myosin-7B antibody; Slow A MYH14 antibody

SEQUENCE SIMILARITIES

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

TISSUE SPECIFICITY

Expressed in heart, skeletal muscle, testis, and all specific brain regions examined. Slightly lower expression was detected in ovary and kidney, and intermediate expression was detected in lung, pancreas, spleen and liver.

DEVELOPMENTAL STAGE

Found in fetal liver and brain.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

The myosin II molecule is a multi-subunit complex consisting of two heavy chains and four light chains. This gene encodes a heavy chain of myosin II, which is a member of the motor-domain superfamily. The heavy chain includes a globular motor domain, which catalyzes ATP hydrolysis and interacts with actin, and a tail domain in which heptad repeat sequences promote dimerization by interacting to form a rod-like alpha-helical coiled coil. This heavy chain subunit is a slow-twitch myosin. Alternatively spliced transcript variants have been found, but the full-length nature of these variants is not determined.