Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Myeloperoxidase Mouse Monoclonal Antibody [A1F2] (EM1901-19)
Immunogen
Recombinant protein within human myeloperoxidase aa 550-745.
Host
Mouse
Positive Control
HL-60 cell, human tonsil tissue, human colon tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A1F2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G purified.
MOLECULAR WEIGHT
84/51 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:2,000-1:5,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Myeloperoxidase
SYNONYMS
84 kDa myeloperoxidase antibody; 89 kDa myeloperoxidase antibody; EC 1.11.1.7 antibody; EC1.11.2.2 antibody; fj80f04 antibody; MPO antibody; mpx antibody; myeloid-specific peroxidase antibody; Myeloperoxidase antibody; Myeloperoxidase heavy chain antibody; Myeloperoxidase light chain antibody; PERM_HUMAN antibody; wu:fj80f04 antibody
SEQUENCE SIMILARITIES
Belongs to the peroxidase family. XPO subfamily.
SUBCELLULAR LOCATION
Lysosome.
FUNCTION
The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue.