PRODUCT CODE: EM1902-38

Myc-tag Mouse Monoclonal Antibody [A3C8] (EM1902-38)

Applications

  • WB

  • ELISA

REACTIVITY

Western blot analysis of Myc-tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Myc-tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Myc-tag on N-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
  • Immobilized N-terminal Myc-tagged recombinant protein. at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.
  • Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.<br />
<br />
Lane 1: Myc Tag fusion protein lysate (input).<br />
Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.<br />
Lane 3: EM31105 IP in Myc Tag fusion protein lysate.<br />
Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate.<br />
<br />
Blocking/Dilution buffer: 5% NFDM/TBST
  • Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.<br />
<br />
Lane 1: Myc Tag fusion protein lysate (input).<br />
Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.<br />
Lane 3: EM31105 IP in Myc Tag fusion protein lysate.<br />
Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate.<br />
<br />
Blocking/Dilution buffer: 5% NFDM/TBST
Western blot analysis of Myc-tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ELISA

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Myc-tag Mouse Monoclonal Antibody [A3C8] (EM1902-38)

Immunogen

Synthetic peptide eqkliseedl conjugated to keyhole limpet haemocyanin.

Host

Mouse

Positive Control

C-terminal Myc-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3C8

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ELISA

  • assay-dependent

TARGET

PROTEIN NAME

Myc-tag

SYNONYMS

AU016757 antibody; Avian myelocytomatosis viral oncogene homolog antibody; bHLHe39 antibody; c Myc antibody; Cellular myelocytomatosis oncogene antibody; Class E basic helix-loop-helix protein 39 antibody; MGC105490 antibody; MRTL antibody; Myc antibody; Myc protein antibody; Myc proto oncogene protein antibody; Myc proto-oncogene protein antibody; myc-related translation/localization regulatory factor antibody; MYC_HUMAN antibody; Myc2 antibody; myca antibody; MYCC antibody; Myelocytomatosis oncogene a antibody; Myelocytomatosis oncogene antibody; Niard antibody; Nird antibody; oncogene c-Myc antibody; Oncogene Myc antibody; OTTHUMP00000158589 antibody; OTTHUMP00000227763 antibody; Proto-oncogene c-Myc antibody; Protooncogene homologous to myelocytomatosis virus antibody; RNCMYC antibody; Transcription factor p64 antibody; Transcriptional regulator Myc-A antibody; V-Myc avian myelocytomatosis viral oncogene homolog antibody; v-myc myelocytomatosis viral oncogene homolog (avian) antibody; zc-myc antibody

FUNCTION

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis . Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).Myc proteins are transcription factors that activate expression of many pro-proliferative genes through binding enhancer box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). Myc is thought to function by upregulating transcript elongation of actively transcribed genes through the recruitment of elongation factors.[8] It can also act as a transcriptional repressor. By binding Miz-1 transcription factor and displacing the p300 co-activator, it inhibits expression of Miz-1 target genes. In addition, myc has a direct role in the control of DNA replication.[9] This activity could contribute to DNA amplification in cancer cells.