PRODUCT CODE: ER50102

MuERVL-Gag Rabbit Polyclonal Antibody (ER50102)

  • Test-Size

Applications

  • ICC

REACTIVITY

  • Mouse

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ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4℃  with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in  red) and phalloidin (shown in green). Nuclear DNA was  labelled with DAPI (shown in blue).
  • ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4℃  with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in  red) and phalloidin (shown in green). Nuclear DNA was  labelled with DAPI (shown in blue).
ICC staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at +4℃ with MuERVL-Gag rabbit pAb at a 1/100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Applications

  • ICC

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MuERVL-Gag Rabbit Polyclonal Antibody (ER50102)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse embryo.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

67 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

MuERVL-Gag

FUNCTION

A large number of retrotransposons are expressed when the zygotic genome is first transcribed, including the endogenous retroviruses (ERVs), LINE-1 elements, and the non-autonomous SINE elements. At the 2C stage, MuERV-L/MERVL retrovirus-like elements are transiently de-repressed and produce 3% of the transcribed mRNAs. Following the 2C stage, MERVL-retroelement expression is silenced. The newly study discovered that this regulated pattern of MERVL expression overlapped with greater than one hundred 2C-specific genes that have co-opted regulatory elements from these foreign retroviruses to initiate their transcription.

CITATIONS

  • Huang, Yun et al.

    Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition. | ELife [2017]

  • Atashpaz, Sina et al.

    ATR expands embryonic stem cell fate potential in response to replication stress. | ELife [2020]