mtTFA Recombinant Rabbit Monoclonal Antibody [JM32-44] (ET1705-64)
Catalog# ET1705-64
Rabbit Monoclonal to mtTFA
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HL-60 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: MCF7 cell lysate (20 µg/Lane)
Lane 6: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 24 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
mtTFA Recombinant Rabbit Monoclonal Antibody [JM32-44] (ET1705-64)
Immunogen
Synthetic peptide within human mttfa aa 41-90 / 246.
Host
Rabbit
Positive Control
K-562 cell lysate, HL-60 cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, Human brain tissue lysate, human kidney tissue, human colon tissue, HeLa, human colon cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM32-44
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 29 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
mtTFA
SYNONYMS
anscription factor 6-like 1 antibody;Mitochondrial transcription factor 1 antibody;mitochondrial transcription factor A antibody;MtTF1 antibody;mtTFA antibody;TCF 6 antibody;TCF-6 antibody;TCF6 antibody;TCF6L1 antibody;TCF6L2 antibody;TCF6L3 antibody;TFAM antibody;TFAM_HUMAN antibody;Transcription factor 6 antibody;Transcription factor 6 like 2 (mitochondrial transcription factor) antibody;Transcription factor 6 like 2 antibody;Transcription factor 6-like 2 antibody;transcription factor 6-like 3 antibody;Transcription factor A, mitochondrial antibody;Transcription factor A, mitochondrial antibody;Transcription factor A, mitochondrial precursor antibody
POST-TRANSLATIONAL MODIFICATION
Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.
SUBCELLULAR LOCATION
Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid.
FUNCTION
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
CITATIONS
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TRAP1 suppresses oral squamous cell carcinoma progression by reducing oxidative phosphorylation metabolism of Cancer-associated fibroblasts
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WB
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Human
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Citation
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Nanxin Zhang,et al
Effects and mechanisms of Polygonati Rhizoma polysaccharide on potassium oxonate and hypoxanthine-induced hyperuricemia in mice
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WB
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Mouse
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Citation
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Knockdown of PGC1α suppresses dysplastic oral keratinocytes proliferation through reprogramming energy metabolism
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WB
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Human
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Citation
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