PRODUCT CODE: ER2001-62

MTHFD2 Rabbit Polyclonal Antibody (ER2001-62)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of MTHFD2 on 293 cell  lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of MTHFD2 on 293 cell  lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of MTHFD2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-62, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of MTHFD2 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MTHFD2 Rabbit Polyclonal Antibody (ER2001-62)

Immunogen

Synthetic peptide within human mthfd2 aa 300-350.

Host

Rabbit

Positive Control

293 cell lysates, Daudi.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MTHFD2

SYNONYMS

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody; Descriptions antibody; Methenyltetrahydrofolate cyclohydrolase antibody; methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody; methylenetetrahydrofolate dehydrogenase 2 antibody; MGC82516 antibody; MTDC_HUMAN antibody; MTHFD2 antibody; mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; NAD-dependent methylene tetrahydrofolate dehydrogenase antibody; NAD-dependent methylenetetrahydrofolate dehydrogenase antibody; NMDMC antibody

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial is an enzyme that in humans is encoded by the MTHFD2 gene. This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.