PRODUCT CODE: ER1902-98

MTHFD2 Rabbit Polyclonal Antibody (ER1902-98)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of MTHFD2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HL-60 cell lysate<br />
Lane 2: 293T cell lysate
  • Western blot analysis of MTHFD2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HL-60 cell lysate<br />
Lane 2: 293T cell lysate
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MTHFD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-98, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MTHFD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using anti-MTHFD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of MTHFD2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-98, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of MTHFD2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: 293T cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MTHFD2 Rabbit Polyclonal Antibody (ER1902-98)

Immunogen

Synthetic peptide within human mthfd2 aa 210-250.

Host

Rabbit

Positive Control

HL-60 cell lysate, 293T cell lysate, human colon carcinoma tissue, mouse kidney tissue, human tonsil carcinoma tissue, Daudi.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • IHC-P:1:50-1:200

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MTHFD2

SYNONYMS

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody; Descriptions antibody; Methenyltetrahydrofolate cyclohydrolase antibody; methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 antibody; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) antibody; methylenetetrahydrofolate dehydrogenase 2 antibody; MGC82516 antibody; MTDC_HUMAN antibody; MTHFD2 antibody; mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody; NAD-dependent methylene tetrahydrofolate dehydrogenase antibody; NAD-dependent methylenetetrahydrofolate dehydrogenase antibody; NMDMC antibody

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial is an enzyme that in humans is encoded by the MTHFD2 gene. This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.