PRODUCT CODE: ER1902-71

MSR1 Rabbit Polyclonal Antibody (ER1902-71)

Applications

  • WB

  • FC

REACTIVITY

  • Human

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Western blot analysis of MSR1 on hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of MSR1 on hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of MSR1 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-71, 1/100) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Western blot analysis of MSR1 on hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MSR1 Rabbit Polyclonal Antibody (ER1902-71)

Immunogen

Recombinant protein corresponding to n terminal human msr1.

Host

Rabbit

Positive Control

Hela cell lysates, SHSY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 49 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MSR1

SYNONYMS

CD204 antibody; CD204 antigen antibody; CD24 antibody; Macrophage acetylated LDL receptor I and II antibody; Macrophage scavenger receptor 1 antibody; Macrophage scavenger receptor type III antibody; Macrophage scavenger receptor types I and II antibody; Msr 1 antibody; MSR1 antibody; MSRE_HUMAN antibody; phSR1 antibody; phSR2 antibody; SCARA 1 antibody; SCARA1 antibody; Scavenger receptor class A member 1 antibody; Scavenger receptor class A, member 1 antibody; Scavenger receptor type A antibody; Scvr antibody; SR A antibody; SRA antibody

TISSUE SPECIFICITY

Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. Isoform I and isoform II are expressed in the liver, placenta and brain.

SUBCELLULAR LOCATION

Membrane

FUNCTION

This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. They were thought to be expressed macrophage-specific, but recently shown to be present on different dendritic cells classes, too. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.