Western blot analysis of MSH6 on A431 cell lysates using anti-MSH6 antibody at 1/1000 dilution.

Applications

WB
ICC
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MSH6 Rabbit Polyclonal Antibody (ER30902)

Immunogen

Synthetic peptide within human msh6aa 1-50 / 1,360.

Host

Rabbit

Positive Control

Hela, A431, Lovo, mouse testicle tissue, rat liver tissue, rat testicle tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

152 kDa

Isotype

IgG

APPLICATION DILUTION

WB
1:1,000

ICC
1:200

IHC-P
1:200

FC
1:100-1:200

TARGET

UNIPROT #

P52701

PROTEIN NAME

MSH6

SYNONYMS

DNA mismatch repair protein Msh6 antibody; G/T mismatch binding protein antibody; G/T mismatch-binding protein antibody; GTBP antibody; GTMBP antibody; hMSH6 antibody; HNPCC 5 antibody; HNPCC5 antibody; HSAP antibody; MSH 6 antibody; MSH6 antibody; MSH6_HUMAN antibody; mutS (E. coli) homolog 6 antibody; MutS alpha 160 kDa subunit antibody; MutS homolog 6 (E. coli) antibody; mutS homolog 6 antibody; MutS-alpha 160 kDa subunit antibody; p160 antibody; Sperm associated protein antibody

SEQUENCE SIMILARITIES

Belongs to the DNA mismatch repair MutS family.

POST-TRANSLATIONAL MODIFICATION

The N-terminus is blocked.; Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

SUBCELLULAR LOCATION

Nucleus, chromosome.

FUNCTION

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage. MSH6 heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world.