MSH2 Mouse Monoclonal Antibody [10G1] (EM1801-04)
Catalog# EM1801-04
Mouse Monoclonal to MSH2
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WB
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IHC-P
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Human
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Rat
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Mouse
Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (EM1801-04) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HEK-293 cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: LNCAP cell lysate (negative) (15 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
MSH2 Mouse Monoclonal Antibody [10G1] (EM1801-04)
Immunogen
Synthetic peptide within n-terminal human msh2.
Host
Mouse
Positive Control
HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, Wild-type SCC7 whole cell lysates, human breast carcinoma tissue, human placenta tissue, rat brain tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
10G1
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IHC-P
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1:100-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 105 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
MSH2
SYNONYMS
BAT26 antibody;COCA 1 antibody;COCA1 antibody;DNA mismatch repair protein Msh2 antibody;FCC 1 antibody;FCC1 antibody;hMSH2 antibody;HNPCC 1 antibody;HNPCC antibody;HNPCC1 antibody;LCFS2 antibody;MSH 2 antibody;Msh2 antibody;MSH2_HUMAN antibody;MutS homolog 2 antibody;MutS homolog 2 colon cancer nonpolyposis type 1 antibody;MutS protein homolog 2 antibody
SEQUENCE SIMILARITIES
Belongs to the DNA mismatch repair MutS family.
TISSUE SPECIFICITY
Ubiquitously expressed.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
SUBCELLULAR LOCATION
Nucleus, Chromosome.
FUNCTION
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
CITATIONS
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Li Y, Zhang Y, Shah SB, Chang CY, Wang H, Wu X
MutSβ protects common fragile sites by facilitating homology-directed repair at DNA double-strand breaks with secondary structures
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WB
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Human
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Citation
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