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Mouse VEGF enzyme-linked immunoassay kit(EM0013)

Price: Regular price $499.00

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Catalog# EM0013

Assay type Sandwich ELISA

Standard curve range 4.12~3000 pg/ml

Sensitivity 0.57 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00840.00910.0088
4.120.02410.02310.02360.0151
12.350.05570.05260.05420.0457
37.040.13730.13370.13550.1270
111.110.37020.35490.36260.3541
333.330.93620.91470.92550.9170
1000.002.07412.02732.05072.0422
3000.003.67623.68253.67943.6709

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)59.4340.6878.464.3347.1993.3
Standard Deviation1.89.821.32.712.120.0
Coefficient of Variation(%)3.02.92.24.23.52.0

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3016.98-45.8710027.58

Sensitivity

0.57 pg/ml (10 μl)

Standard curve range

4.12~3000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse VEGF into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 114% to 131% with an overall mean recovery of 122%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse VEGF enzyme-linked immunoassay kit(EM0013)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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