Mouse TNF-α enzyme-linked immunoassay kit(EM0010)
Catalog# EM0010
Assay type Sandwich ELISA
Standard curve range 1.37~1000 pg/ml
Sensitivity 0.097 pg/ml (10 μl)
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Mouse
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0121 | 0.0111 | 0.0116 | |
| 1.37 | 0.0242 | 0.0223 | 0.0233 | 0.0117 |
| 4.12 | 0.0482 | 0.0444 | 0.0463 | 0.0347 |
| 12.35 | 0.1134 | 0.1092 | 0.1113 | 0.0997 |
| 37.04 | 0.3165 | 0.3032 | 0.3099 | 0.2983 |
| 111.11 | 0.8042 | 0.7996 | 0.8019 | 0.7903 |
| 333.33 | 1.9672 | 1.9564 | 1.9618 | 1.9502 |
| 1000 | 4.1683 | 4.2715 | 4.2199 | 4.2083 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 16.1 | 80.4 | 271.7 | 14 | 75.2 | 236.7 |
| Standard Deviation | 1.1 | 4.8 | 16 | 0.3 | 4 | 10.8 |
| Coefficient of Variation(%) | 6.5 | 5.9 | 5.9 | 2 | 5.3 | 4.6 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.28 | 33 | 0.16 |
Sensitivity
0.097 pg/ml (10 μl)
Standard curve range
1.37~1000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of mouse TNF alpha into mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 107% to 132% with an overall mean recovery of 116%.
Specifications
Product Type
ELISA Kit
Product Name
Mouse TNF-α enzyme-linked immunoassay kit(EM0010)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
CITATIONS
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Yu Jinjin,et al
(−)-Epicatechin gallate prevented atherosclerosis by reducing abnormal proliferation of VSMCs and oxidative stress of AML 12 cells
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ELISA
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Mouse
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Citation
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