Mouse MCSF/CSF-1, C-His Tag (ECD) Protein (Active) (HA210725)
Catalog# HA210725
Biologically active: yes
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Mouse
Product Details
Product Type
Protein
Product Name
Mouse MCSF/CSF-1, C-His Tag (ECD) Protein (Active) (HA210725)
Predicted molecular mass
27.4 kD
Protein construction description
A DNA sequence encoding the mouse MCSF/CSF-1 protein (P07141) (Lys 33-Glu 262) was expressed with a His tag at the C-terminus.
Accession
P07141
Protein construction
MCSF/CSF-1 (33-262) | His
Source
HEK293
Bio Activity
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is 0.5-3 ng/mL
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Mouse
Background
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Usage Guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
CITATIONS
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Rapamycin Inhibits Osteoclastogenesis and Prevents LPS-Induced Alveolar Bone Loss by Oxidative Stress Suppression
