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Mouse Leptin enzyme-linked immunoassay kit (EM0027)

Price: Regular price $499.00

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Catalog# EM0027

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 19.30 pg/ml (50 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01670.01680.0168
312.500.09510.09620.09570.0789
625.000.17740.17350.17550.1587
1250.000.33330.33940.33640.3196
2500.000.62850.62690.62770.6110
5000.001.20101.19101.19601.1793
10000.002.17002.12602.14802.1313
20000.003.53403.62203.57803.5613

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)427.51982.75614.0422.21991.26030.1
Standard Deviation12.348.3268.17.781.3142.6
Coefficient of Variation(%)2.92.44.81.84.12.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum302244.18-16867.461008181.3

Sensitivity

19.30 pg/ml (50 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse Leptin into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 72% to 119% with an overall mean recovery of 97%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse Leptin enzyme-linked immunoassay kit (EM0027)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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