Mouse IL-6 enzyme-linked immunoassay kit(EM0004)
Catalog# EM0004
Assay type Sandwich ELISA
Standard curve range 8.19~2000 pg/ml
Sensitivity 0.36 pg/ml (10 μl)
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Mouse
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0176 | 0.0182 | 0.0179 | |
| 8.19 | 0.0346 | 0.0364 | 0.0355 | 0.0176 |
| 20.48 | 0.0681 | 0.0668 | 0.0675 | 0.0496 |
| 51.20 | 0.1466 | 0.1447 | 0.1457 | 0.1278 |
| 128.00 | 0.3521 | 0.3347 | 0.3434 | 0.3255 |
| 320.00 | 0.8173 | 0.8337 | 0.8255 | 0.8076 |
| 800.00 | 1.7570 | 1.7990 | 1.7780 | 1.7601 |
| 2000.00 | 3.3660 | 3.4510 | 3.4085 | 3.3906 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 40.7 | 199.7 | 642.4 | 37.3 | 177.3 | 585.0 |
| Standard Deviation | 1.2 | 7.1 | 23.6 | 1.7 | 5.3 | 17.7 |
| Coefficient of Variation(%) | 3.0 | 3.6 | 3.7 | 4.5 | 3.0 | 3.0 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-16.26 | 93.3 | 4.35 |
Sensitivity
0.36 pg/ml (10 μl)
Standard curve range
8.19~2000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-6 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 95% with an overall mean recovery of 85%.
Specifications
Product Type
ELISA Kit
Product Name
Mouse IL-6 enzyme-linked immunoassay kit(EM0004)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
CITATIONS
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Bao J, Wang Z, Yang Y, et al
Interleukin-17 alleviates erastin-induced alveolar bone loss by suppressing ferroptosis via interaction between NRF2 and p-STAT3
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ELISA
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Mouse
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Citation
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