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Mouse IL-2 enzyme-linked immunoassay kit(EM0002)

Price: Regular price $499.00

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Catalog# EM0002

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 0.16 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.010.0090.0095
2.740.01820.01820.01820.0087
8.230.0310.0310.0310.0215
24.690.0720.07360.07280.0633
74.070.18370.19180.18780.1783
222.220.52830.51490.52160.5121
666.671.41511.44131.42821.4187
20003.46573.4093.43743.4279

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)43.5121.4362.018.9152.5341.9
Standard Deviation2.26.019.40.42.812.5
Coefficient of Variation(%)5.15.05.42.11.83.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-0.67210.23

Sensitivity

0.16 pg/ml (10 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 108% with an overall mean recovery of 94%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-2 enzyme-linked immunoassay kit(EM0002)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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