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Mouse IL-1β enzyme-linked immunoassay kit(EM0029)

Price: Regular price $499.00

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Catalog# EM0029

Assay type Sandwich ELISA

Standard curve range 15.63~1000 pg/ml

Sensitivity 0.91 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.06510.07010.0676
15.630.11040.11430.11240.0448
31.250.16600.16090.16350.0959
62.500.26230.26760.26500.1974
125.000.47400.46500.46950.4019
250.000.85930.83160.84550.7779
500.001.50701.60601.55651.4889
1000.002.77802.80802.79302.7254

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)18.993.0290.217.497.5280.7
Standard Deviation1.04.516.92.38.514.6
Coefficient of Variation(%)5.34.95.86.16.15.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-3.66131.08

Sensitivity

0.91 pg/ml (10 μl)

Standard curve range

15.63~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-1 beta into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 111% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-1β enzyme-linked immunoassay kit(EM0029)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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