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Mouse IL-10 enzyme-linked immunoassay kit(EM0005)

Price: Regular price $499.00

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Catalog# EM0005

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 5.75 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02070.02090.0208
13.720.03330.03110.03220.0114
41.150.05860.05530.05700.0362
123.460.11520.112150.11360.0928
370.370.30110.29840.29980.2790
1111.110.77950.77760.77860.7578
3333.331.99811.90361.95051.9297
10000.003.71313.71123.71203.6912

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)179.9784.52838.4218.4918.72985.2
Standard Deviation6.234.5140.311.058.2166.5
Coefficient of Variation(%)3.54.44.95.06.46.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3019.31-941.79100120.83

Sensitivity

5.75 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-10 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 84% to 119% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-10 enzyme-linked immunoassay kit(EM0005)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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