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Mouse IFN-γ enzyme-linked immunoassay kit(EM0009)

Price: Regular price $499.00

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Catalog# EM0009

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 3.24 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.04710.04800.0476
13.720.06050.05600.05830.0107
41.150.08400.07580.07990.0324
123.460.16370.14740.15560.1080
370.370.37650.33450.35550.3080
1111.110.97900.89600.93750.8900
3333.332.29382.23302.26342.2159
10000.004.28104.70314.49214.4445

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)158.31074.02844.2167.91116.92911.3
Standard Deviation7.359.9139.78.858.2158.0
Coefficient of Variation(%)4.65.64.95.25.25.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.0n.d.

Sensitivity

3.24 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IFN-γ into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 93% to 121% with an overall mean recovery of 101%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IFN-γ enzyme-linked immunoassay kit(EM0009)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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