Mouse CXCL2/MIP-2 enzyme-linked immunoassay kit(EM0018)
Catalog# EM0018
Assay type Sandwich ELISA
Standard curve range 13.72~10000 pg/ml
Sensitivity 10.2 pg/ml (10 μl)
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Mouse
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0130 | 0.0122 | 0.0126 | |
| 13.72 | 0.0151 | 0.0142 | 0.0147 | 0.0021 |
| 41.15 | 0.0210 | 0.0210 | 0.0210 | 0.0084 |
| 123.46 | 0.0381 | 0.0411 | 0.0396 | 0.0270 |
| 370.37 | 0.1251 | 0.1312 | 0.1282 | 0.1156 |
| 1111.11 | 0.5023 | 0.5012 | 0.5018 | 0.4892 |
| 3333.33 | 1.7271 | 1.8113 | 1.7692 | 1.7566 |
| 10000.00 | 3.9910 | 4.0931 | 4.0421 | 4.0295 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 160.2 | 1021.4 | 3095.3 | 153.9 | 1034.9 | 3264.4 |
| Standard Deviation | 8.9 | 64.2 | 148.8 | 9.0 | 55.2 | 180.8 |
| Coefficient of Variation(%) | 5.6 | 6.3 | 4.8 | 5.9 | 5.3 | 5.5 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d. | 0 | n.d. |
Sensitivity
10.2 pg/ml (10 μl)
Standard curve range
13.72~10000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL2/MIP-2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 89% to 114% with an overall mean recovery of 101%.
Specifications
Product Type
ELISA Kit
Product Name
Mouse CXCL2/MIP-2 enzyme-linked immunoassay kit(EM0018)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
CITATIONS
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Fuxiang Li,et al
SIRT7 remodels the cytoskeleton via RAC1 to enhance host resistance to Mycobacterium tuberculosis
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Elisa
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Mouse;Human
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Citation
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