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Mouse CCL5 / RANTES enzyme-linked immunoassay kit (EM0032)

Price: Regular price $499.00

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Catalog# EM0032

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.86 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01090.01200.0115
6.860.02220.02290.02260.0111
20.580.04900.04790.04850.0370
61.730.11530.10770.11150.1001
185.190.33410.32220.32820.3167
555.561.06201.01301.03751.0261
1666.672.88602.84102.86352.8521
5000.004.34894.33044.33974.3282

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)111.7647.31815.5107.4516.01760.2
Standard Deviation7.443.1130.26.625.886.3
Coefficient of Variation(%)6.66.77.26.15.04.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3015.09-64.5610037.12

Sensitivity

1.86 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse CCL5 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 99% to 125% with an overall mean recovery of 116%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse CCL5 / RANTES enzyme-linked immunoassay kit (EM0032)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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