HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Mouse Monoclonal Antibody [A11-G11] (M1007-11)
Catalog# M1007-11
Mouse Monoclonal to Mouse anti-Human IgG light chain(kappa chain)
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WB
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ELISA
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Human
Specifications
Product Type
Mouse monoclonal primary
Product Name
HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Mouse Monoclonal Antibody [A11-G11] (M1007-11)
Immunogen
Human igg light chain
Host
Mouse
Positive Control
Human IgG
Conjugation
Hrp
Clonality
Monoclonal
Clone Number
A11-G11
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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ELISA
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
25 kDa
Isotype
IgG2a
TARGET
PROTEIN NAME
Mouse anti-Human IgG light chain(kappa chain)
SUBCELLULAR LOCATION
Serum/ Plasma
FUNCTION
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
CITATIONS
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Potent anti-tumor activity of CD45RA-targeting Hm3A4-Ranpirnase against myeloid lineage leukemias
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WB
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Human
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Citation
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