Applications
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WB
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ICC
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IF
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IHC-P
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IP
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FC
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MMP14 Recombinant Rabbit Monoclonal Antibody [SJ18-09] (ET1606-48)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Human kidney tissue lysates, CRC, BT-20, human breast carcinoma tissue, human kidney tissue, human uterus tissue, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SJ18-09
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
65 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:1,000
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ICC/IF
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
MMP14
SYNONYMS
Matrix metallopeptidase 14 (membrane inserted) antibody; Matrix metalloproteinase 14 antibody; Matrix metalloproteinase-14 antibody; Membrane type 1 matrix metalloproteinase antibody; Membrane type 1 metalloprotease antibody; Membrane type matrix metalloproteinase 1 antibody; Membrane-type matrix metalloproteinase 1 antibody; Membrane-type-1 matrix metalloproteinase antibody; MMP 14 antibody; MMP X1 antibody; MMP-14 antibody; MMP-X1 antibody; Mmp14 antibody; MMP14_HUMAN antibody; MMPX1 antibody; MT MMP 1 antibody; MT-MMP 1 antibody; MT1 MMP antibody; MT1-MMP antibody; MT1MMP antibody; MTMMP 1 antibody; MTMMP1 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M10A family.
TISSUE SPECIFICITY
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
POST-TRANSLATIONAL MODIFICATION
The precursor is cleaved by a furin endopeptidase.; Tyrosine phosphorylated by PKDCC/VLK.
SUBCELLULAR LOCATION
Membrane, Melanosome, Cytoplasm.
FUNCTION
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.
CITATIONS
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Lin, Yinuo et al.
Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells. | Journal of Extracellular Vesicles [2020]
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WB
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human
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Citation
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