PRODUCT CODE: ER31211

MMP1 Rabbit Polyclonal Antibody (ER31211)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.<br />
Positive control:   <br />
Lane 1: SKBR3           <br />
Lane 2: Raji <br />
Lane 3: A431           <br />
Lane 4: A549
  • Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.<br />
Positive control:   <br />
Lane 1: SKBR3           <br />
Lane 2: Raji <br />
Lane 3: A431           <br />
Lane 4: A549
  • ICC staining MMP1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP1 antibody. Counter stained with hematoxylin.
Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.
Positive control:
Lane 1: SKBR3
Lane 2: Raji
Lane 3: A431
Lane 4: A549

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MMP1 Rabbit Polyclonal Antibody (ER31211)

Immunogen

Synthetic peptide within human mmp1 aa 432-475.

Host

Rabbit

Positive Control

A549, MCF-7, Raji, SKBR3, A431, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

MMP1

SYNONYMS

27 kDa interstitial collagenase antibody; CLG antibody; CLGN antibody; collagenase, fibroblast antibody; collagenase, interstitial antibody; Fibroblast collagenase antibody; Interstitial collagenase antibody; Matrix metallopeptidase 1 (interstitial collagenase) antibody; Matrix metalloprotease 1 antibody; Matrix Metalloproteinase 1 antibody; Matrix metalloproteinase-1 antibody; MMP 1 antibody; MMP-1 antibody; MMP1 antibody; MMP1_HUMAN antibody; OTTHUMP00000045866 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M10A family.

POST-TRANSLATIONAL MODIFICATION

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.; Tyrosine phosphorylated in platelets by PKDCC/VLK.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

MMP-1 has an archetypal structure consisting of a pre-domain, a pro-domain, a catalytic domain, a linker region and a hemopexin-like domain. Two main nomenclatures for the primary structure are currently in use, the original one from which the first amino-acid starts with the signalling peptide and a second one where the first amino-acid starts counting from the prodomain (proenzyme nomenclature). MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Specifically, MMP-1 breaks down the interstitial collagens, types I, II, and III. Mechanical force may increase the expression of MMP1 in human periodontal ligament cells.

CITATIONS

  • Wang, Ji et al.

    Matrix metalloproteinase-1 expression in breast carcinoma: a marker for unfavorable prognosis. | Oncotarget [2017]