PRODUCT CODE: ER1706-77

MMP-3 Rabbit Polyclonal Antibody (ER1706-77)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.
  • Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.
  • ICC staining MMP-3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MMP-3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with MMP-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MMP-3 Rabbit Polyclonal Antibody (ER1706-77)

Immunogen

Recombinant protein within c-terminal human mmp-3.

Host

Rabbit

Positive Control

A549, LOVO, rat brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MMP-3

SYNONYMS

CHDS6 antibody; Matrix metalloproteinase 3 antibody; Matrix metalloproteinase-3 antibody; MGC126102 antibody; MGC126103 antibody; MGC126104 antibody; MMP 3 antibody; MMP-3 antibody; MMP3 antibody; MMP3_HUMAN antibody; Proteoglycanase antibody; SL-1 antibody; SL1 antibody; STMY antibody; STMY1 antibody; STR1 antibody; Stromelysin 1 antibody; Stromelysin-1 antibody; Transin 1 antibody; Transin-1 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M10A family.

SUBCELLULAR LOCATION

Extracellular matrix. Secreted.

FUNCTION

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, -2 and -3 respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.