PRODUCT CODE: HA600034

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

  • ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.
  • The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.
  • Down ELISA Binding Assay of anti-MMAF Antibody A5G12(HA600034) to MMAF-ADC and MMAF-BSA. The mouse mAb works fine with ELISA assay for measuring MMAF derivative ADC.
The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.

Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

Applications

  • ELISA

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Immunogen

Mmaf coupled with ova.

Host

Mouse

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A5G12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

Isotype

IgG1

APPLICATION DILUTION

  • ELISA

  • 1:5,000-1:20,000

TARGET

PROTEIN NAME

MMAF

SYNONYMS

MMAF antibody Monomethyl auristatin F antibdy

FUNCTION

Monomethyl auristatin F (MMAF) is a synthetic antineoplastic agent. It is part of the approved drug belantamab mafodotin in multiple myeloma and some experimental anti-cancer antibody-drug conjugates such as vorsetuzumab mafodotin and SGN-CD19A. In International Nonproprietary Names for MMAF-antibody-conjugates, the name mafodotin refers to MMAF plus its attachment structure to the antibody. Monomethyl auristatin F is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. It is linked to an antibody with high affinity to structures on cancer cells, causing MMAF to accumulate in such cells. MMAF is actually desmethyl-auristatin F; that is, the N-terminal amino group has only one methyl substituent instead of two as in auristatin F itself.