PRODUCT CODE: ET1601-25

MLKL Recombinant Rabbit Monoclonal Antibody [SA40-04] (ET1601-25)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of MLKL on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HT-29 cell lysate<br />
Lane 2: HUVEC cell lysate<br />
Lane 3: Hela cell lysate
  • Western blot analysis of MLKL on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HT-29 cell lysate<br />
Lane 2: HUVEC cell lysate<br />
Lane 3: Hela cell lysate
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLKL antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of MLKL on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HT-29 cell lysate
Lane 2: HUVEC cell lysate
Lane 3: Hela cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MLKL Recombinant Rabbit Monoclonal Antibody [SA40-04] (ET1601-25)

Immunogen

Recombinant protein within human mlkl aa 333-471 / 471.

Host

Rabbit

Positive Control

HUVEC, HT29, Hela, HepG2, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SA40-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

MLKL

SYNONYMS

9130019I15Rik antibody; FLJ34389 antibody; hMLKL antibody; Mixed lineage kinase domain like antibody; Mixed lineage kinase domain like protein antibody; Mixed lineage kinase domain-like protein antibody; Mlkl antibody; MLKL_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

SUBCELLULAR LOCATION

Cytoplasm, Cell membrane, Golgi apparatus.

FUNCTION

MLKL (mixed lineage kinase domain-like) is a 471 amino acid protein that contains one protein kinase domain which is thought to be catalytically inactive. The gene encoding MLKL maps to chromosome 16 and is expressed as two isoforms which are produced by alternative splicing events. Chromosome 16, which is associated with a variety of genetic disorders, encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.