Mitofusin 2 Recombinant Rabbit Monoclonal Antibody [JE59-66] (HA720073)
Catalog# HA720073
Rabbit Monoclonal to Mitofusin 2
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WB
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 75 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720073) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Mitofusin 2 Recombinant Rabbit Monoclonal Antibody [JE59-66] (HA720073)
Immunogen
Synthetic peptide within human mitofusin 2 aa 51-100/757.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, Mouse brain tissue lysate, HepG2 cell lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE59-66
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 86 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Mitofusin 2
SYNONYMS
CMT2A antibody;CMT2A2 antibody;CPRP 1 antibody;CPRP1 antibody;EC 3.6.5.- antibody;Fzo antibody;HSG antibody;hyperplasia suppressor gene antibody;Hypertension related protein 1 antibody;KIAA0214 antibody;MARF antibody;MFN 2 antibody;Mfn2 antibody;MFN2_HUMAN antibody;Mitochondrial assembly regulatory factor antibody;Mitofusin-2 antibody;Mitofusin2 antibody;Transmembrane GTPase MFN2 antibody
SUBCELLULAR LOCATION
Mitochondrion outer membrane.
FUNCTION
Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains. Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
CITATIONS
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Zhang Honghao,et al
Targeting PARP14 with lomitapide suppresses drug resistance through the activation of DRP1-induced mitophagy in multiple myeloma
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WB
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Human
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Citation
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Jingyu Zhang,et al
Nr-CWS regulates METTL3-mediated m6A modification of CDS2 mRNA in vascular endothelial cells and has prognostic significance
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WB
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Human
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Citation
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Zeng Chaiming,et al
Silencing MFN2 Drives WNT/β-catenin Nucleation to Reduce Sorafenib Sensitivity in Hepatocellular Carcinoma Cells
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WB
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Human
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Citation
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