PRODUCT CODE: ET1703-89

MIF Recombinant Rabbit Monoclonal Antibody [JM11-64] (ET1703-89)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.<br />
Positive control: <br />
  Line 1: Mouse brain <br />
  Line 2: Jurkat <br />
  Line 3: Hela
  • Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.<br />
Positive control: <br />
  Line 1: Mouse brain <br />
  Line 2: Jurkat <br />
  Line 3: Hela
  • Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.
Positive control:
Line 1: Mouse brain
Line 2: Jurkat
Line 3: Hela

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MIF Recombinant Rabbit Monoclonal Antibody [JM11-64] (ET1703-89)

Immunogen

Synthetic peptide within human mif aa 60-90.

Host

Rabbit

Positive Control

Jurkat, Hela, THP-1, mouse brain.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-64

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

12 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

MIF

SYNONYMS

GIF antibody; GLIF antibody; Glycosylation inhibiting factor antibody; Glycosylation-inhibiting factor antibody; L-dopachrome isomerase antibody; L-dopachrome tautomerase antibody; Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody; Macrophage migration inhibitory factor antibody; MIF antibody; MIF protein antibody; MIF_HUMAN antibody; MMIF antibody; Phenylpyruvate tautomerase antibody

SEQUENCE SIMILARITIES

Belongs to the MIF family.

SUBCELLULAR LOCATION

Secreted. Cytoplasm.

FUNCTION

Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor α (TNFα), interleukin-1 (IL-1) and interferon-γ (IFN-γ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.