Midkine Recombinant Rabbit Monoclonal Antibody [JF096-5] (ET1702-64)
Catalog# ET1702-64
Rabbit Monoclonal to Midkine
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Midkine on different lysates with Rabbit anti-Midkine antibody (ET1702-64) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: U-2 OS cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: U-2 OS cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-64) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.