MICU2 Recombinant Rabbit Monoclonal Antibody [PSH01-84] (HA721742)
Catalog# HA721742
Rabbit Monoclonal to MICU2
Tested for
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WB
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IHC-P
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IF-Tissue
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FC
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MICU2 on different lysates with Rabbit anti-MICU2 antibody (HA721742) at 1/1,000 dilution.
Lane 1: LNCaP cell lysate, 30 µg/Lane.
Lane 2: NIH/3T3 cell lysate, 30 µg/Lane.
Lane 3: PC-12 cell lysate, 30 µg/Lane.
Lane 4: Human kidney tissue lysate, 30 µg/Lane.
Lane 5: Human liver tissue lysate, 30 µg/Lane.
Lane 6: Mouse heart tissue lysate, 30 µg/Lane.
Lane 7: Mouse kidney tissue lysate, 30 µg/Lane.
Lane 8: Rat kidney tissue lysate, 30 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 42 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721742) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: LNCaP cell lysate, 30 µg/Lane.
Lane 2: NIH/3T3 cell lysate, 30 µg/Lane.
Lane 3: PC-12 cell lysate, 30 µg/Lane.
Lane 4: Human kidney tissue lysate, 30 µg/Lane.
Lane 5: Human liver tissue lysate, 30 µg/Lane.
Lane 6: Mouse heart tissue lysate, 30 µg/Lane.
Lane 7: Mouse kidney tissue lysate, 30 µg/Lane.
Lane 8: Rat kidney tissue lysate, 30 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 42 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721742) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.