mGluR3 Recombinant Rabbit Monoclonal Antibody [JE41-18] (HA722434)
Catalog# HA722434
Rabbit Monoclonal to mGluR3
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of mGluR3 on different lysates with Rabbit anti-mGluR3 antibody (HA722434) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 99 kDa
Observed band size: 99-250 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722434) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 99 kDa
Observed band size: 99-250 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722434) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.