Mesothelin Recombinant Rabbit Monoclonal Antibody [PSH02-30] (HA721810)
Catalog# HA721810
Rabbit Monoclonal to Mesothelin
Tested for
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (HA721810) at 1/1,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: NCI-H226 cell lysate
Lane 3: HeLa cell lysate
Lane 4: NIH:OVCAR-3 cell lysate
Lane 5: PC-3M cell lysate (negative)
Lane 6: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 40 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (HA721810) at 1/1,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: NCI-H226 cell lysate
Lane 3: HeLa cell lysate
Lane 4: NIH:OVCAR-3 cell lysate
Lane 5: PC-3M cell lysate (negative)
Lane 6: A549 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 40 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721810) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.