Melanoma gp100 Recombinant Rabbit Monoclonal Antibody [JE42-47] (HA721719)
Catalog# HA721719
Rabbit Monoclonal to Melanoma gp100
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721719) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Melanoma gp100 Recombinant Rabbit Monoclonal Antibody [JE42-47] (HA721719)
Immunogen
Synthetic peptide within human melanoma gp100 aa 617-661 / 661.
Host
Rabbit
Positive Control
SK-MEL-28 cell lysates, human melanoma tissue, mouse testis tissue, SK-MEL-28.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE42-47
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 70 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Melanoma gp100
SYNONYMS
95 kDa melanocyte specific secreted glycoprotein antibody;95 kDa melanocyte-specific secreted glycoprotein antibody;D12S53E antibody;gp100 antibody;M-beta antibody;ME20 antibody;ME20 M/ME20 S antibody;ME20-M antibody;ME20-S antibody;ME20M antibody;ME20M/ME20S antibody;ME20S antibody;Melanocyte lineage specific antigen GP100 antibody;Melanocyte protein mel 17 antibody;Melanocyte protein Pmel 17 antibody;Melanocyte protein Pmel 17 precursor antibody;Melanocytes lineage-specific antigen GP100 antibody;Melanoma associated ME20 antigen antibody;Melanoma gp100 antibody;Melanoma-associated ME20 antigen antibody;Melanosomal matrix protein 17 antibody;Melanosomal matrix protein17 antibody;P1 antibody;p100 antibody;p26 antibody;PMEL 17 antibody;PMEL antibody;PMEL_HUMAN antibody;PMEL17 antibody;Premelanosome protein antibody;Secreted melanoma-associated ME20 antigen antibody;SI antibody;SIL antibody;SILV antibody;Silver (mouse homolog) like antibody;Silver homolog antibody;Silver locus protein homolog antibody;Silver, mouse, homolog of antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Golgi apparatus, cis-Golgi network membrane, Endosome, multivesicular body, Melanosome, Extracellular vesicle, Secreted.
FUNCTION
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
CITATIONS
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Yu Jinhai,et al
Three-Dimensional Cell Spheroid Culture and Proliferation Activity Study of Uveal Melanoma Cell Line C918
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IHC
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Human
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Citation
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