PRODUCT CODE: ET1610-47

MelanA Recombinant Rabbit Monoclonal Antibody [SC56-02] (ET1610-47)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of MelanA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Melanoma tissue lysate<br />
Lane 2: B18F1 cell lysate
  • Western blot analysis of MelanA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Melanoma tissue lysate<br />
Lane 2: B18F1 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human melanoma tissue using anti-MelanA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of MelanA was done on B16F1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-47, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of MelanA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Melanoma tissue lysate
Lane 2: B18F1 cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MelanA Recombinant Rabbit Monoclonal Antibody [SC56-02] (ET1610-47)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Melanoma tissue lysate, B18F1 cell lysate, human melanoma tissue, B16F1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC56-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

MelanA

SYNONYMS

Antigen LB39 AA antibody; Antigen LB39-AA antibody; Antigen SK29 AA antibody; Antigen SK29-AA antibody; MAR1_HUMAN antibody; MART 1 antibody; MART-1 antibody; MART1 antibody; Melan A antibody; Melan A protein antibody; Melanoma antigen recognized by T cells 1 antibody; Melanoma antigen recognized by T-cells 1 antibody; MLAN A antibody; MLANA antibody; OTTHUMP00000021036 antibody; OTTHUMP00000021037 antibody; OTTHUMP00000021038 antibody; Protein Melan-A antibody

TISSUE SPECIFICITY

Expression is restricted to melanoma and melanocyte cell lines and retina.

POST-TRANSLATIONAL MODIFICATION

Acylated.

SUBCELLULAR LOCATION

Golgi apparatus, trans-Golgi network membrane, Endoplasmic reticulum membrane, Melanosome.

FUNCTION

Melanoma-associated antigens recognized by cytotoxic T lymphocytes (CTL) have been grouped into three categories: melanocyte differentiation antigens, cancer/testis-specific antigens and mutated or aberrantly expressed antigens. Many of these antigens consist of peptides that are presented to T cells by HLA molecules; they represent potential targets for cancer immunotherapy. Melan-A (also designated MART-1) is a melanocyte differentiation antigen that is specific to melanomas, melanocyte cell lines and retina. Melan-A peptide is recognized by most HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes in patients with melanoma. Antimelanoma cytotoxic T lymphocytes can be generated with a Melan-A peptide, implicating Melan-A as a potential candidate for antigen-specific immunotherapy in melanoma patients.