PRODUCT CODE: ET1610-70

MEKK2 Recombinant Rabbit Monoclonal Antibody [SC68-02] (ET1610-70)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: HepG2            <br />
Lane 2: Rat brain <br />
Lane 3: SW480
  • Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: HepG2            <br />
Lane 2: Rat brain <br />
Lane 3: SW480
  • ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Rat brain
Lane 3: SW480

Applications

  • WB

  • ICC

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MEKK2 Recombinant Rabbit Monoclonal Antibody [SC68-02] (ET1610-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SW480, MCF-7, HepG2, mouse brain tissue, human breast carcinoma tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC68-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MEKK2

SYNONYMS

M3K2_HUMAN antibody; Map3k2 antibody; MAPK/ERK kinase kinase 2 antibody; MEK kinase 2 antibody; MEKK 2 antibody; MEKK2b antibody; Mitogen activated protein kinase kinase kinase 2 antibody; Mitogen-activated protein kinase kinase kinase 2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated.; Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.