MDM2 Rabbit Polyclonal Antibody (ER1902-14)
Catalog# ER1902-14
Rabbit Polyclonal to MDM2
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WB
-
IF-Cell
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IHC-P
-
FC
-
Human
Positive control:
Lane 1: human lung tissue lysate
Lane 2: Daudi cell lysate
Specifications
Product Type
Rabbit polyclonal primary
Product Name
MDM2 Rabbit Polyclonal Antibody (ER1902-14)
Immunogen
Synthetic peptide within human mdm2 aa 1-50 / 491.
Host
Rabbit
Positive Control
Human lung tissue lysate, Daudi cell lysate, HepG2, JAR, MCF-7, human thyroid tissue, human skin tissue, human breast carcinoma tissue, human small intestine tissue, human pancreas tissue, Daudi.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB:1:500-1:1,000
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IF-Cell:1:50-1:200
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IHC-P:1:500-1:1,000
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FC:1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MDM2
SYNONYMS
ACTFS antibody;Double minute 2 protein antibody;E3 ubiquitin-protein ligase Mdm2 antibody;Hdm 2 antibody;Hdm2 antibody;HDMX antibody;MDM 2 antibody;MDM2 antibody;MDM2 oncogene E3 ubiquitin protein ligase antibody;Mdm2 p53 E3 ubiquitin protein ligase homolog antibody;Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody;MDM2_HUMAN antibody;MDM2BP antibody;Mouse Double Minute 2 antibody;MTBP antibody;Murine Double Minute Chromosome 2 antibody;Oncoprotein Mdm2 antibody;p53 Binding Protein Mdm2 antibody;p53-binding protein Mdm2 antibody;Ubiquitin protein ligase E3 Mdm2 antibody;Ubiquitin protein ligase E3 Mdm2 antibody
SEQUENCE SIMILARITIES
Belongs to the MDM2/MDM4 family.
TISSUE SPECIFICITY
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53. Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this prevents oligomerization and E3 ligase processivity and impedes constitutive p53/TP53 degradation.; Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN. Also ubiquitinated by TRIM13. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53. Deubiquitinated by USP7 leading to its stabilization.
SUBCELLULAR LOCATION
Nucleoplasm, nucleolus, cytoplasm.
FUNCTION
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
