Applications
-
WB
REACTIVITY
-
Mouse
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Mcur1 Rabbit Polyclonal Antibody (ER1902-30)
Immunogen
Synthetic peptide within mouse mcur1 79-128 / 340.
Host
Rabbit
Positive Control
Mouse brain tissue lysates.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
38/32/19 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
TARGET
UNIPROT #
PROTEIN NAME
Mcur1
SYNONYMS
Mitochondrial calcium uniporter regulator antibody; MCU regulator 1 antibody; Coiled-coil domain-containing protein 90A, mitochondrial antibody; Mcur1 antibody; Ccdc90a antibody
SEQUENCE SIMILARITIES
Belongs to the CCDC90 family.
SUBCELLULAR LOCATION
Membrane, Mitochondrion, Mitochondrion inner membrane。
FUNCTION
Ca2+ flux across the mitochondrial inner membrane regulates bioenergetics, cytoplasmic Ca2+ signals and activation of cell death pathways. Mitochondrial Ca2+ uptake occurs at regions of close apposition with intracellular Ca2+ release sites driven by the inner membrane voltage generated by oxidative phosphorylation and mediated by a Ca2+ selective ion channel called the uniporter. Mitochondrial calcium uniporter (MCU) was recently identified as an ion-conducting pore. MCUR1 (mitochondrial calcium uniporter regulator 1), belongs to the CCDC90 family, an integral membrane protein required for MCU-dependent mitochondrial Ca2+ uptake. MCUR1 binds to MCU and regulates ruthenium-red-sensitive MCU-dependent Ca2+ uptake. MCUR1 contains two transmembrane helices, where the N- and the short C-termini are exposed to the inter-membrane space. MCUR1 is ubiquitously expressed in mammalian tissues including: skeletal muscle, brain, heart, lung, liver, kidney, large intenstine and spleen. It was found that cells depleted of MCUR1 have abnormal bioenergetic properties similar to those observed in cells in which Ca2+ uptake is blocked. Thus, MCUR1 is necessary for MCU to efficiently promote mitochondrial Ca2+ uptake and to ensure normal mitochondrial bioenergetics.