MCU Rabbit Polyclonal Antibody (ER1803-57)
Catalog# ER1803-57
Rabbit Polyclonal to MCU
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
Positive control:
Lane 1: A549 cell lysate
Lane 3: mouse spleen tissue lysate
Lane 2: HL-60 cell lysate
Lane 4: mouse brain tissue lysate
Specifications
Product Type
Rabbit polyclonal primary
Product Name
MCU Rabbit Polyclonal Antibody (ER1803-57)
Immunogen
Recombinant protein within human mcu aa 51-225 / 351.
Host
Rabbit
Positive Control
A549 cell lysate, HL-60 cell lysate, mouse spleen tissue lysates, mouse brain tissue lysates, HT-29, rat brain tissue, human colon tissue, mouse testis tissue, A549.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:50
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 40 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MCU
SYNONYMS
C109A_HUMAN antibody;C10orf42 antibody;Calcium uniporter protein mitochondrial antibody;Ccdc109a antibody;Coiled-coil domain-containing protein 109A antibody;HsMCU antibody;Mitochondrial Calcium Uniporter antibody
SEQUENCE SIMILARITIES
Belongs to the MCU (TC 1.A.77) family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation by CaMK2 in heart leads to increased MCU current. The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results. Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex. Activity is regulated by MICU1 and MICU2. At low Ca2+ levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca2+ levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells.
CITATIONS
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Zhong, C., Yang, J., Zhang, Y., Fan, X., Fan, Y., Hua, N., Li, D., Jin, S., Li, Y., Chen, P., Chen, Y., Cai, X., Zhang, Y., Jiang, L., Yang, W., Yu, P., & Lin, H.
TRPM2 Mediates Hepatic Ischemia-Reperfusion Injury via Ca2+-Induced Mitochondrial Lipid Peroxidation through Increasing ALOX12 Expression
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WB
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Mouse
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Citation
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