Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse colon tissue lysate
Applications
-
WB
-
IHC-P
-
ICC
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
MCM7 Rabbit Polyclonal Antibody (ER1902-60)
Immunogen
Recombinant protein corresponding to c terminal human mcm7.
Host
Rabbit
Positive Control
MCF-7 cell lysate, K562 cell lysate, mouse colon tissue lysate, MCF-7, MG-63, SHSY5Y, human lung cancer tissue, human colon tissue, mouse skin tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
Predicted band size 81/60/45 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
ICC
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
MCM7
SYNONYMS
CDABP0042 antibody; CDC 47 antibody; CDC47 antibody; CDC47 homolog antibody; Cdc47, S. cerevisiae, homolog of antibody; DNA replication licensing factor MCM7 antibody; Homolog of S. cerevisiae Cdc47 antibody; MCM 2 antibody; MCM 7 antibody; MCM2 antibody; MCM2, formerly antibody; Mcm7 antibody; MCM7 minichromosome maintenance deficient 7 antibody; MCM7_HUMAN antibody; Minichromosome Maintainence 7 antibody; Minichromosome maintainence, S. cerevisiae, homolog of antibody; Minichromosome maintenance complex component 7 antibody; Minichromosome maintenance deficient 7 antibody; Minichromosome maintenance protein 7 antibody; P1.1 MCM3 antibody; P1.1-MCM3 antibody; P1CDC47 antibody; P85MCM antibody; PNAS 146 antibody; PNAS146 antibody; PPP1R104 antibody; Protein phosphatase 1, regulatory subunit 104 antibody
SEQUENCE SIMILARITIES
Belongs to the MCM family.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.