Western blot analysis of MCM7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: HL-60 cell lysate
Lane 2: 293 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: K562 cell lysate
Mouse monoclonal primary
MCM7 Mouse Monoclonal Antibody [15E1] (EM1901-12)
Recombinant protein within human mcm7 aa 516-719 / 719.
HL-60 cell, 293 cell, PC-12 cell, K562 cell, human tonsil tissue, human esophagus tissue, SH-SY5Y cell.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.