PRODUCT CODE: EM1901-12

MCM7 Mouse Monoclonal Antibody [15E1] (EM1901-12)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of MCM7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HL-60 cell lysate<br />
Lane 2: 293 cell lysate<br />
Lane 3: PC-12 cell lysate<br />
Lane 4: K562 cell lysate
  • Western blot analysis of MCM7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HL-60 cell lysate<br />
Lane 2: 293 cell lysate<br />
Lane 3: PC-12 cell lysate<br />
Lane 4: K562 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-12, 1/100)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-12, 1/100)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of MCM7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of MCM7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: 293 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: K562 cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

MCM7 Mouse Monoclonal Antibody [15E1] (EM1901-12)

Immunogen

Recombinant protein within human mcm7 aa 516-719 / 719.

Host

Mouse

Positive Control

HL-60 cell, 293 cell, PC-12 cell, K562 cell, human tonsil tissue, human esophagus tissue, SH-SY5Y cell.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

15E1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

81 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • IHC-P:1:50-1:200

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

MCM7

SYNONYMS

CDABP0042 antibody; CDC 47 antibody; CDC47 antibody; CDC47 homolog antibody; Cdc47, S. cerevisiae, homolog of antibody; DNA replication licensing factor MCM7 antibody; Homolog of S. cerevisiae Cdc47 antibody; MCM 2 antibody; MCM 7 antibody; MCM2 antibody; MCM2, formerly antibody; Mcm7 antibody; MCM7 minichromosome maintenance deficient 7 antibody; MCM7_HUMAN antibody; Minichromosome Maintainence 7 antibody; Minichromosome maintainence, S. cerevisiae, homolog of antibody; Minichromosome maintenance complex component 7 antibody; Minichromosome maintenance deficient 7 antibody; Minichromosome maintenance protein 7 antibody; P1.1 MCM3 antibody; P1.1-MCM3 antibody; P1CDC47 antibody; P85MCM antibody; PNAS 146 antibody; PNAS146 antibody; PPP1R104 antibody; Protein phosphatase 1, regulatory subunit 104 antibody

SEQUENCE SIMILARITIES

Belongs to the MCM family.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.