MCM5 Recombinant Rabbit Monoclonal Antibody [JB31-34] (ET7107-15)
Catalog# ET7107-15
Rabbit Monoclonal to MCM5
Tested for
-
WB
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IHC-P
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MCM5 on different lysates with Rabbit anti-MCM5 antibody (ET7107-15) at 1/500 dilution.
Lane 1: SiHa cell lysate
Lane 2: HL-60 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: SiHa cell lysate
Lane 2: HL-60 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.