MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04] (ET1606-14)
Catalog# ET1606-14
Rabbit Monoclonal to MCL1
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
Western blot analysis of MCL1 on different lysates with Rabbit anti-MCL1 antibody (ET1606-14) at 1/1,000 dilution.
Lane 1: MCF7-si NT cell lysate
Lane 2: MCF7-si MCL1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04] (ET1606-14)
Immunogen
Synthetic peptide within human mcl1 aa 101-150 / 350.
Host
Rabbit
Positive Control
Raji cell lysate, Ramos cell lysate, MCF7 cell lysate, SK-OV-3 cell lysate, mouse heart tissue lysates, Hela, HepG2, BT-20, human breast tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SI16-04
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MCL1
SYNONYMS
Bcl 2 related protein EAT/mcl1 antibody;Bcl-2-like protein 3 antibody;Bcl-2-related protein EAT/mcl1 antibody;BCL2 related antibody;Bcl2-L-3 antibody;BCL2L3 antibody;EAT antibody;Induced myeloid leukemia cell differentiation protein Mcl 1 antibody;Induced myeloid leukemia cell differentiation protein Mcl-1 antibody;MCL 1 antibody;MCL1 antibody;MCL1-ES antibody;mcl1/EAT antibody;MCL1_HUMAN antibody;MCL1L antibody;MCL1S antibody;MGC104264 antibody;MGC1839 antibody;Myeloid Cell Leukemia 1 antibody;Myeloid cell leukemia ES antibody;Myeloid cell leukemia sequence 1 antibody;Myeloid cell leukemia sequence 1 BCL2 related antibody;Myeloid cell leukemia sequence 1 isoform 1 antibody;OTTHUMP00000032794 antibody;OTTHUMP00000032795 antibody;TM antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
POST-TRANSLATIONAL MODIFICATION
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.; Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.; Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.; Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.
SUBCELLULAR LOCATION
Membrane, Cytoplasm, Mitochondrion, Nucleus.
FUNCTION
B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.
CITATIONS
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