Mature Neuron Marker Antibody Kit (HAK21007)
Catalog# HAK21007
Reacts with
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Human
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Mouse
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Rat
Western blot analysis of NeuN on different lysates with Rabbit anti-NeuN antibody (ET1602-12) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)
Lane 4: Rat cerebellum tissue lysate (20 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 45/50 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-12) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane)
Lane 4: Rat cerebellum tissue lysate (20 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 45/50 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-12) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.