Western blot analysis of MAT2A on rat kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500028, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Rabbit polyclonal primary
MAT2A Rabbit Polyclonal Antibody (HA500028)
Recombinant protein within human mat2a aa 70-260.
Rat kidney tissue lysates, rat kidney tissue, human colon tissue, human colon carcinoma tissue, mouse brain tissue, Hela.
Protein expression is regulated by post-transcriptional regulation: in presence of S-adenosyl-L-methionine, METTL16 binds and methylates the first hairpin of the 3'-UTR region of MAT2A mRNA, impairing MAT2A expression . In S-adenosyl-L-methionine-limiting conditions, METTL16 binds the 3'-UTR region of MAT2A mRNA without methylating it due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A . Consequences of N6-methylation of MAT2A are however subject to discussion: According to a report, N6-methylation of MAT2A affects MAT2A mRNA splicing ). According to a second report, N6-methylation of MAT2A affects MAT2A mRNA stability (By similarity).By similarity1 Publication.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.