PRODUCT CODE: ET1610-64

Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [SC68-07] (ET1610-64)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Mast Cell Tryptase on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Mast Cell Tryptase on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-64, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-64, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Mast Cell Tryptase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Mast Cell Tryptase on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [SC68-07] (ET1610-64)

Immunogen

Recombinant protein within human mast cell tryptase aa 10-275 / 275.

Host

Rabbit

Positive Control

Human skin tissue lysates, human tonsil tissue, human small intestine tissue, human prostate carcinoma tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC68-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

30 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Mast Cell Tryptase

SYNONYMS

alpha II antibody; Lung tryptase antibody; Mast cell alpha II tryptase antibody; Mast cell beta I tryptase antibody; Mast cell protease 7 antibody; Mast cell protease II antibody; MCP 7 antibody; Pituitary tryptase antibody; Skin tryptase antibody; TPS 1 antibody; TPS1 antibody; TPS2 antibody; TPSAB1 antibody; TPSAB1 protein antibody; TPSB1 antibody; Tryptase 1 antibody; Tryptase alpha 1 antibody; tryptase alpha I included antibody; Tryptase alpha II antibody; tryptase alpha II included antibody; tryptase alpha included antibody; tryptase alpha/beta 1 antibody; Tryptase beta 1 antibody; tryptase beta I included antibody; Tryptase I antibody; tryptase I included antibody; Tryptase III antibody; Tryptase skin antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S1 family. Tryptase subfamily.

TISSUE SPECIFICITY

Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin; in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level).

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast cell chymase, known as CMA1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19-amino acid signal peptide, an acidic 2-amino acid propeptide and a 226-amino acid catalytic domain. Tryptases comprise a family of trypsin-like serine proteases that are enzymatically active as heparin-stabilized tetramers. There are four functional genes for tryptase: α I, β I, β II and γ I, which map to human chromosome 16p13.3, with β tryptases representing the main isoenzymes expressed in mast cells. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function.

CITATIONS

  • Wang, J., Guo, X., Chen,......

    Wang, J., Guo, X., Chen, C., Sun, S., Liu, G., Liu, M., Hao, M., & Che, H. (2021). Gender differences in food allergy depend on the PPAR γ/NF-κB in the intestines of mice. Life sciences, 278, 119606.