MARK2 Recombinant Rabbit Monoclonal Antibody [JE40-97] (ET7109-20)
Catalog# ET7109-20
Rabbit Monoclonal to MARK2
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: A549-WT cell lysate
Lane 2: A549-KD MARK2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 75/80 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
MARK2 Recombinant Rabbit Monoclonal Antibody [JE40-97] (ET7109-20)
Immunogen
Recombinant protein within human mark2 aa 661-789 / 789.
Host
Rabbit
Positive Control
A549 cell lysate, Raji cell lysate, U-87 MG cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human appendix tissue, Raji.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE40-97
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 88 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MARK2
SYNONYMS
ELKL motif kinase 1 antibody;ELKL motif kinase antibody;EMK-1 antibody;EMK1 antibody;MAP/microtubule affinity regulating kinase 2 antibody;MAP/microtubule affinity-regulating kinase 2 antibody;Mark2 antibody;MARK2_HUMAN antibody;MGC99619 antibody;PAR 1 antibody;Par 1b antibody;PAR1 homolog antibody;Par1b antibody;Ser/Thr protein kinase PAR 1B antibody;Serine/threonine protein kinase EMK antibody;Serine/threonine protein kinase MARK2 antibody;Serine/threonine-protein kinase MARK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
TISSUE SPECIFICITY
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm.
SUBCELLULAR LOCATION
Cell membrane. Cytoplasm. Cytoskeleton.
FUNCTION
Microtubule affinity-regulating kinase 2 (MARK2), also known as EMK1 (ELKL motif kinase 1) or Par1b, is a 788 amino acid protein that is a member of the protein kinase superfamily, MARK subfamily. Highly expressed in heart, brain, skeletal muscle and pancreas, MARK2 is essential for the asymmetric development of membrane domains around polarized epithelial cells. Activation of MARK2 by phosphorylation on Thr 208 allows the protein to modulate the building of a columnar versus a hepatic epithelial cell. MARK2 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. MARK2 is expressed as 14 isoforms produced by alternative splicing events. Some of these isoforms may function in graft rejection.
CITATIONS
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Jianmin Li
MARK2 enhances cisplatin resistance via PI3K/AKT/ NF-κB signaling pathway in osteosarcoma cells
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IHC
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Human
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Citation
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