MAPK6 / ERK3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-39] (HA750873)
Catalog# HA750873
Rabbit Monoclonal to MAPK6 / ERK3
Tested for
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WB
-
IF-Cell
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FC
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of MAPK6 / ERK3 on different lysates with Rabbit anti-MAPK6 / ERK3 antibody (HA750873) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: PC-3M cell lysate
Lane 5: C2C12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 105 kDa
Exposure time: 5 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750873) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: PC-3M cell lysate
Lane 5: C2C12 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 105 kDa
Exposure time: 5 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750873) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.